Identification of blood meal of field caught Aedes aegypti (L.) by multiplex PCR.
نویسندگان
چکیده
Laboratory bred female Aedes aegypti (L.) was used to determine sensitivity of multiplex PCR for detecting human blood meal. Human blood DNA was detected in live fully fed mosquitoes until 3 days after blood feeding, and for 4 weeks when stored at -20 degrees C. Among 890 field caught female mosquito samples examined for vertebrate DNA by multiplex PCR, results were positive for human, pig, dog, cow and mixture of 2 host DNA at 86.1, 3.4, 2.1, 1.0 and 3.6%, respectively, while 3.9% of the samples were negative. Blood feeding pattern must be considered when mosquito control strategies become employed.
منابع مشابه
Detection of dengue viruses in field caught male Aedes aegypti and Aedes albopictus (Diptera: Culicidae) in Singapore by type-specific PCR.
Field male Aedes aegypti (L.) and Aedes albopictus (Skuse) adults caught from fixed monitoring stations weekly for 1 yr were screened for dengue viruses (DEN-1, DEN-2, DEN-3, and DEN-4). The assay was carried out using a single-step reverse transcription (or transcriptase)-polymerase chain reaction (PCR) (RT-PCR) followed by a semi-nested PCR using an upstream consensus primer and four type-spe...
متن کاملTransmission of African Horse-Sickness by Means of Mosquito Bites and Replication of the Virus in Aedes aegypti
متن کامل
An Aedes aegypti-associated fungus increases susceptibility to dengue virus by modulating gut trypsin activity
Transmission of dengue virus (DENV) requires successful completion of the infection cycle in the Aedes aegypti vector, which is initiated in the midgut tissue after ingestion of an infectious blood meal. While certain Ae. aegypti midgut-associated bacteria influence virus infection, little is known about the midgut-associated fungi (mycobiota), and how its members might influence susceptibility...
متن کاملLaboratory Evaluation Of Mosquito Traps Baited With A Synthetic Human Odor Blend To Capture <i>Aedes Aegypti</i>
A synthetic blend of chemicals comprising volatiles released by the human body has been shown to be an effective attractant for female Aedes aegypti in olfactometer bioassays with laboratory-reared mosquitoes. We report the laboratory evaluation of Ae. aegypti response to a synthetic blend tested with 4 types of mosquito traps (CDC model 512, CDC model 1012, CFG, and Fay–Prince traps). Aedes ae...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- The Southeast Asian journal of tropical medicine and public health
دوره 41 1 شماره
صفحات -
تاریخ انتشار 2010