Identification of blood meal of field caught Aedes aegypti (L.) by multiplex PCR.

نویسندگان

  • Padet Siriyasatien
  • Theerakamol Pengsakul
  • Veerayuth Kittichai
  • Atchara Phumee
  • Sakchai Kaewsaitiam
  • Usavadee Thavara
  • Apiwat Tawatsin
  • Preecha Asavadachanukorn
  • Mir S Mulla
چکیده

Laboratory bred female Aedes aegypti (L.) was used to determine sensitivity of multiplex PCR for detecting human blood meal. Human blood DNA was detected in live fully fed mosquitoes until 3 days after blood feeding, and for 4 weeks when stored at -20 degrees C. Among 890 field caught female mosquito samples examined for vertebrate DNA by multiplex PCR, results were positive for human, pig, dog, cow and mixture of 2 host DNA at 86.1, 3.4, 2.1, 1.0 and 3.6%, respectively, while 3.9% of the samples were negative. Blood feeding pattern must be considered when mosquito control strategies become employed.

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عنوان ژورنال:
  • The Southeast Asian journal of tropical medicine and public health

دوره 41 1  شماره 

صفحات  -

تاریخ انتشار 2010